| Title: | Proteomic profiling and scRNA sequencing identify signatures associated with Helicobacter pylori infection and risk of developing gastric cancer |
| Journal: | Cancer Biology & Medicine |
| Published: | 4 Sep 2025 |
| Pubmed: | https://pubmed.ncbi.nlm.nih.gov/40923140/ |
| DOI: | https://doi.org/10.20892/j.issn.2095-3941.2025.0077 |
| URL: | https://www.cancerbiomed.org/content/cbm/22/8/946.full.pdf |
Publication 19572
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Abstract
OBJECTIVE: The key molecular events signifying the Helicobacter pylori-induced gastric carcinogenesis process are largely unknown.</p>
METHODS: Bulk tissue-proteomics profiling were leveraged across multi-stage gastric lesions from Linqu (n = 166) and Beijing sets (n = 99) and single-cell transcriptomic profiling (n = 18) to decipher key molecular signatures of H. pylori-related gastric lesion progression and gastric cancer (GC) development. The association of key proteins association with gastric lesion progression and GC development were prospectively studied building on follow-up of the Linqu set and UK Biobank (n = 48,529).</p>
RESULTS: Concordant proteomics signatures associated with H. pylori infection and gastric carcinogenesis (ρ = 0.784, correlation P = 1.80 × 10-36) were identified. RNA expression of genes encoding 13 up- and 15 down-regulated key proteins displayed trending alterations in the transition from normal gastric epithelium to intestinal metaplasia, then to malignant cells. A 15-tissue protein panel integrating these signatures demonstrated potential for targeting individuals at high risk for progressing to gastric neoplasia (OR = 7.22, 95% CI: 1.31-39.72 for the high-score group). A 4-circulating protein panel may be used as non-invasive markers predicting the risk of GC development (hazard ratio = 3.73, 95% confidence interval: 1.63-8.54, high-risk vs. low-risk populations, area under the curve = 0.75).</p>
CONCLUSIONS: Concordant proteomics signatures associated with H. pylori infection and gastric carcinogenesis were unveiled with potential as biomarkers for targeted prevention strategies.</p>
13 Keywords
- Aged
- Biomarkers, Tumor
- Female
- Gene Expression Profiling
- Helicobacter Infections
- Helicobacter pylori
- Humans
- Male
- Middle Aged
- Proteome
- Proteomics
- Single-Cell Analysis
- Stomach Neoplasms
10 Authors
- Yu Jin
- Xue Li
- Bingyao Cai
- Lanxin Yang
- Wenjing Zhao
- Hengmin Xu
- Yang Zhang
- Zongchao Liu
- Kaifeng Pan
- Wenqing Li
1 Application
| Application ID | Title |
|---|---|
| 90999 | Integrating metabolomic characteristics, genetic predisposition, and host factors to decipher the risk of gastric cancer |
Enabling scientific discoveries that improve human health